目前蛋白质组学耗材成本高昂。
现有蛋白质组学方法需要很高的技术门槛。
需要建立简单易用和随时可用的方法。
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Empore™ E系列技术简介
E3™技术特点
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高效：手动操作时间<15min； 稳定：与多种细胞裂解条件兼容（例如：尿素、SDS、RIPA、TFA等）； 经济：弥补基因组学和蛋白质组学产品之间的差距； 易用：对入门级生物医学工作者零技术障碍； 多样：E3tips™、E3filter™和E3plate™可满足不同的样品体积、浓度、数量和自动化的需求； 简便：无需液体转移，无需考虑任何与游离磁珠相关的问题（蛋白与磁珠的比例、磁珠粘附在管壁和表面、交叉污染等）； Empore™产品服务于蛋白组学领域20多年，有深厚的应用基础。
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E3™产品类型
E3filter测试结果
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Fig.1 Qualitative and quantitative assessment of E3technology (E3 filter) for E. coli proteome analysis. (A-C) Comparison of the number of proteins, peptides, and PSMs between the E3filter, FASP, and SP4-GB approaches. Error bars represent three replicates. (D-E) Overlapping analyses of proteins and peptides derived from the three methods. (F) Coefficient of variation of quantified proteins by the three methods. (G) Coefficient of variation of quantified peptides. (H) Pearson correlation between replicate experiments and different methods. (I) Percentages of missed cleavages. (J-K) Volcano plot of showing significantly differential proteins between the three methods. The two curves show FDR 0.05 and 0.01, respectively. (L) Heatmap of the overall quantified proteins by the three methods. FASP, filter aided sample preparation; SP4, Solvent Precipitation SP3; GB, glassbeads.
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E3工作流程
E4工作流程
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参考文献
Yu YB, et al. Cell Reports Methods 4, 2024, 100796, June 17.
Johnston HE, et al.Anal Chem.2022, 94:10320-10328.
WiśniewskiJR, et al. Nat Methods. 2009,6:359-62.
